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EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer.

ABSTRACT: BACKGROUND:Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. METHODS:In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. RESULTS:210 miRNAs were significantly dysregulated (adjusted p value?

SUBMITTER: Roman-Canal B

PROVIDER: S-EPMC6585099 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer.

Roman-Canal Berta B Tarragona Jordi J Moiola Cristian Pablo CP Gatius Sònia S Bonnin Sarah S Ruiz-Miró Maria M Sierra José Enrique JE Rufas Maria M González Esperanza E Porcel José M JM Gil-Moreno Antonio A Falcón-Pérez Juan M JM Ponomarenko Julia J Matias-Guiu Xavier X Colas Eva E

Journal of translational medicine 20190620 1

<h4>Background</h4>Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk.<h4>Methods</h4>In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the ...[more]

PMID: 31221189

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Project description:BackgroundExtracellular vesicles (EVs), ubiquitously released by blood cells, facilitate intercellular communication. In cancer, tumor-derived EVs profoundly affect the microenvironment, promoting tumor progression and raising the risk of recurrence. These EVs contain miRNAs (EV-miRNAs), promising cancer biomarkers. Characterizing plasma EVs and identifying EV-miRNAs associated with breast cancer recurrence are crucial aspects of cancer research since they allow us to discover new biomarkers that are effective for understanding tumor biology and for being used for early detection, disease monitoring, or approaches to personalized medicine. This study aimed to characterize plasma EVs in breast cancer (BC) patients and identify EV-miRNAs associated with BC recurrence.MethodsThis retrospective observational study included 24 BC patients divided into recurrence (n= 11) and non-recurrence (n= 13) groups. Plasma EVs were isolated and characterized. Total RNA from EVs was analyzed for miRNA expression using NanoString's nCounter® miRNA Expression Assays panel. MicroRNA target prediction used mirDIP, and pathway interactions were assessed via Reactome.ResultsA stronger presence of circulating EVs was found to be linked with a less favorable prognosis (p = 0.0062). We discovered a distinct signature of EV-miRNAs, notably including miR-19a-3p and miR-130b-3p, which are significantly associated with breast cancer recurrence. Furthermore, miR-19a-3p and miR-130b-3p were implicated in the regulation of PTEN and MDM4, potentially contributing to breast cancer progression.A notable association emerged, indicating a high concentration of circulating EVs predicts poor prognosis (p = 0.0062). Our study found a distinct EV-miRNA signature involving miR-19a-3p and miR-130b-3p, strongly associated with disease recurrence. We also presented compelling evidence for their regulatory roles in PTEN and MDM4 genes, contributing to BC development.ConclusionThis study revealed that increased plasma EV concentration is associated with BC recurrence. The prognostic significance of EVs is closely tied to the unique expression profiles of miR-19a-3p and miR-130b-3p. These findings underscore the potential of EV-associated miRNAs as valuable indicators for BC recurrence, opening new avenues for diagnosis and treatment exploration.

Dataset Information

EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer.

Publications

EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer.

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