A novel method to establish glucocorticoid resistant acute lymphoblastic leukemia cell lines.

ABSTRACT:

Background

Drug-resistant cell lines, established from drug-sensitive cell lines by drug exposure in vitro, are the most useful cancer models in studies on the mechanism of chemoresistance. However, the success rate of the traditional approaches to construct such cell lines is low because a long time is required for the addition of drugs.

Methods

A cell culture technique was used to establish the drug-resistant cell lines from their parental cells. Molecular and cellular biological techniques including flow cytometry, MTT assay, western blotting, and DNA fingerprinting analysis were used to characterize the drug-resistant cell lines. Nude mice were used for xenograft studies.

Results

We established novel glucocorticoid (GC)-resistant cell lines from 3 GC-sensitive acu

SUBMITTER: Gu L 

PROVIDER: S-EPMC6585113 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature