In Silico Serotyping Based on Whole-Genome Sequencing Improves the Accuracy of Shigella Identification.

ABSTRACT: Bacteria of the genus Shigella, consisting of 4 species and >50 serotypes, cause shigellosis, a foodborne disease of significant morbidity, mortality, and economic loss worldwide. Classical Shigella identification based on selective media and serology is tedious, time-consuming, expensive, and not always accurate. A molecular diagnostic assay does not distinguish Shigella at the species level or from enteroinvasive Escherichia coli (EIEC). We inspected genomic sequences from 221 Shigella isolates and observed low concordance rates between conventional designation and molecular serotyping: 86.4% and 80.5% at the species and serotype levels, respectively. Serotype determinants for 6 additional serotypes were identified. Examination of differentiation gene m

SUBMITTER: Wu Y 

PROVIDER: S-EPMC6585509 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature