Unknown

Dataset Information

0

A Versatile and Convenient Synthesis of 34 S-Labeled Phosphorothioate Oligonucleotides.


ABSTRACT: A synthetic protocol for 34 S-labeled phosphorothioate oligonucleotides (PS ONs) was developed to facilitate MS-based assay analysis. This was enabled by a highly efficient, two-step, one-pot synthesis of 34 S-labeled phenylacetyl disulfide (34 S-PADS), starting from 34 S-enriched elemental sulfur (34 S8 ). 34 S-PADS was subsequently used for stable isotope labeling (SIL) of oligonucleotides containing a phosphorothioate backbone. The 34 S-SIL PS ONs are shown to retain the same melting temperature, antisense activity, and secondary structure as those of the corresponding unlabeled 32 S PS ONs.

SUBMITTER: Stulz R 

PROVIDER: S-EPMC6585993 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6571627 | biostudies-literature
| S-EPMC5537454 | biostudies-literature
| S-EPMC10067960 | biostudies-literature
| S-EPMC4267618 | biostudies-literature
| S-EPMC6316614 | biostudies-literature
| S-EPMC6264524 | biostudies-literature
| S-EPMC25326 | biostudies-literature
| S-EPMC11229082 | biostudies-literature
| S-EPMC5333924 | biostudies-literature
| S-EPMC9116672 | biostudies-literature