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A parallelized microfluidic DNA bisulfite conversion module for streamlined methylation analysis.

ABSTRACT: Aberrant methylation of DNA has been identified as an epigenetic biomarker for numerous cancer types. The vast majority of techniques aimed at detecting methylation require bisulfite conversion of the DNA sample prior to analysis, which until now has been a benchtop process. Although microfluidics has potential benefits of simplified operation, sample and reagent economy, and scalability, bisulfite conversion has yet to be implemented in this format. Here, we present a novel droplet microfluidic design that facilitates rapid bisulfite conversion by reducing the necessary processing steps while retaining comparable performance to existing methods. This new format has a reduced overall processing time and is readily scalable for use in high throughput DNA methylation analysis.

SUBMITTER: Stark A

PROVIDER: S-EPMC6586411 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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A parallelized microfluidic DNA bisulfite conversion module for streamlined methylation analysis.

Stark Alejandro A Shin Dong Jin DJ Pisanic Thomas T Hsieh Kuangwen K Wang Tza-Huei TH

Biomedical microdevices 20160201 1

Aberrant methylation of DNA has been identified as an epigenetic biomarker for numerous cancer types. The vast majority of techniques aimed at detecting methylation require bisulfite conversion of the DNA sample prior to analysis, which until now has been a benchtop process. Although microfluidics has potential benefits of simplified operation, sample and reagent economy, and scalability, bisulfite conversion has yet to be implemented in this format. Here, we present a novel droplet microfluidic ...[more]

PMID: 26759004

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