ESCRT-dependent vacuolar sorting and degradation of the auxin biosynthetic enzyme YUC1 flavin monooxygenase.
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ABSTRACT:
SUBMITTER: Ge C
PROVIDER: S-EPMC6588437 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Publications
ESCRT-dependent vacuolar sorting and degradation of the auxin biosynthetic enzyme YUC1 flavin monooxygenase.
Ge Chennan C Gao Caiji C Chen Qingguo Q Jiang Liwen L Zhao Yunde Y
Journal of integrative plant biology 20190319 9
YUC flavin monooxygenases catalyze the rate-limiting step of auxin biosynthesis. Here we report the vacuolar targeting and degradation of GFP-YUC1. GFP-YUC1 fusion expressed in Arabidopsis protoplasts or transgenic plants was primarily localized in vacuoles. Surprisingly, we established that GFP-YUC1, a soluble protein, was sorted to vacuoles through the ESCRT pathway, which has long been recognized for sorting and targeting integral membrane proteins. We further show that GFP-YUC1 was ubiquitin ...[more]
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