Application of CRISPR/Cas9-Based Genome Editing in Studying the Mechanism of Pandrug Resistance in Klebsiella pneumoniae.
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ABSTRACT:
SUBMITTER: Sun Q
PROVIDER: S-EPMC6591634 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Publications
Application of CRISPR/Cas9-Based Genome Editing in Studying the Mechanism of Pandrug Resistance in Klebsiella pneumoniae.
Sun Qiaoling Q Wang Yu Y Dong Ning N Shen Lingwei L Zhou Hongwei H Hu Yanyan Y Gu Danxia D Chen Sheng S Zhang Rong R Ji Quanjiang Q
Antimicrobial agents and chemotherapy 20190624 7
In this study, a CRISPR/Cas9-mediated genome editing method was used to study the functions of the <i>mgrB</i>, <i>tetA</i>, and <i>ramR</i> genes in mediating colistin and tigecycline resistance in carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP). Inactivation of the <i>tetA</i> or <i>ramR</i> gene or the <i>mgrB</i> gene by CRISPR/Cas9 affected bacterial susceptibility to tigecycline or colistin, respectively. This study proved that the CRISPR/Cas9-based genome editing method could be ...[more]
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