E2A attenuates tumor-initiating capacity of colorectal cancer cells via the Wnt/beta-catenin pathway.

ABSTRACT:

Background

The E2A gene, which encodes two basic helix-loop-helix transcription factors, E12 and E47, regulates colorectal cancer progression and epithelial-mesenchymal transition. However, whether E2A regulates the tumor-initiating capacity of colorectal cancer is unclear. Thus, we have studied E2A expression in the initiation of colorectal cancer in vivo and in vitro.

Methods

Immunohistochemistry and immunoblot were performed to determine protein levels of E2A in colorectal cancer specimens and cells. RNAi was employed to downregulate E2A expression, and the subsequent change in protein level was evaluated by immunoblot. Sphere-forming assay and enumeration of liver metastasis in mouse models were used to identify the tumor formation ability of colorectal cancer cells.

SUBMITTER: Zhao H 

PROVIDER: S-EPMC6591938 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature