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Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles.

ABSTRACT: Antifreeze proteins (AFPs) have many potential applications, ranging from cryobiology to aerospace, if they can be incorporated into materials. Here, a range of engineered AFP mutants were prepared and site-specifically conjugated onto RAFT polymer-stabilized gold nanoparticles to generate new hybrid multivalent ice growth inhibitors. Only the SNAP-tagged AFPs lead to potent 'antifreeze' active nanomaterials with His-Tag capture resulting in no activity, showing the mode of conjugation is essential. This versatile strategy will enable the development of multivalent AFPs for translational and fundamental studies.

SUBMITTER: Wilkins LE

PROVIDER: S-EPMC6592154 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles.

Wilkins Laura E LE Hasan Muhammad M Fayter Alice E R AER Biggs Caroline C Walker Marc M Gibson Matthew I MI

Polymer chemistry 20190131 23

Antifreeze proteins (AFPs) have many potential applications, ranging from cryobiology to aerospace, if they can be incorporated into materials. Here, a range of engineered AFP mutants were prepared and site-specifically conjugated onto RAFT polymer-stabilized gold nanoparticles to generate new hybrid multivalent ice growth inhibitors. Only the SNAP-tagged AFPs lead to potent 'antifreeze' active nanomaterials with His-Tag capture resulting in no activity, showing the mode of conjugation is essent ...[more]

PMID: 31303900

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