Ontology highlight
ABSTRACT:
SUBMITTER: Chen L
PROVIDER: S-EPMC4989145 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Chen Long L Cohen Justin J Song Xiaoda X Zhao Aishan A Ye Zi Z Feulner Christine J CJ Doonan Patrick P Somers Will W Lin Laura L Chen Peng R PR
Scientific reports 20160818
Sortase mediated ligation is a highly specific platform for conjugation that relies on the specificity of the transpeptidase Sortase A (SrtA) for short peptide sequences (LPXTG and GGG). SrtA retains its specificity while accepting a wide range of potential substrates, but its broad use is limited by the wild-type enzyme's poor kinetics, which require large amounts of SrtA and extended reaction times for efficient conjugation. Prior explorations have aimed to improve the kinetics of SrtA with li ...[more]