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Broadly resistant HIV-1 against CD4-binding site neutralizing antibodies.


ABSTRACT: Recently identified broadly neutralizing antibodies (bnAbs) show great potential for clinical interventions against HIV-1 infection. However, resistant strains may impose substantial challenges. Here, we report on the identification and characterization of a panel of HIV-1 strains with broad and potent resistance against a large number of bnAbs, particularly those targeting the CD4-binding site (CD4bs). Site-directed mutagenesis revealed that several key epitope mutations facilitate resistance and are located in the inner domain, loop D, and ?23/loop V5/?24 of HIV-1 gp120. The resistance is largely correlated with binding affinity of antibodies to the envelope trimers expressed on the cell surface. Our results therefore demonstrate the existence of broadly resistant HIV-1 strains against CD4bs neutralizing antibodies. Treatment strategies based on the CD4bs bnAbs must overcome such resistance to achieve optimal clinical outcomes.

SUBMITTER: Zhou P 

PROVIDER: S-EPMC6592578 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Broadly resistant HIV-1 against CD4-binding site neutralizing antibodies.

Zhou Panpan P   Wang Han H   Fang Mengqi M   Li Yangyang Y   Wang Hua H   Shi Shasha S   Li Zihao Z   Wu Jiapeng J   Han Xiaoxu X   Shi Xuanling X   Shang Hong H   Zhou Tongqing T   Zhang Linqi L  

PLoS pathogens 20190613 6


Recently identified broadly neutralizing antibodies (bnAbs) show great potential for clinical interventions against HIV-1 infection. However, resistant strains may impose substantial challenges. Here, we report on the identification and characterization of a panel of HIV-1 strains with broad and potent resistance against a large number of bnAbs, particularly those targeting the CD4-binding site (CD4bs). Site-directed mutagenesis revealed that several key epitope mutations facilitate resistance a  ...[more]

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