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A mechanism for preventing asymmetric histone segregation onto replicating DNA strands.

ABSTRACT: How parental histone (H3-H4)2 tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands of DNA replication forks for epigenetic inheritance remains elusive. Here we show that parental (H3-H4)2 tetramers are assembled into nucleosomes onto both leading and lagging strands, with a slight preference for lagging strands. The lagging-strand preference increases markedly in budding yeast cells lacking Dpb3 and Dpb4, two subunits of the leading strand DNA polymerase, Pol ?, owing to the impairment of parental (H3-H4)2 transfer to leading strands. Dpb3-Dpb4 binds H3-H4 in vitro and participates in the inheritance of heterochromatin. These results indicate that different proteins facilitate the transfer of parental (H3-H4)2 onto leading versus lagging strands and that Dbp3-Dpb4 plays an important role in this poorly understood process.

SUBMITTER: Yu C

PROVIDER: S-EPMC6597248 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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A mechanism for preventing asymmetric histone segregation onto replicating DNA strands.

Yu Chuanhe C Gan Haiyun H Serra-Cardona Albert A Zhang Lin L Gan Songlin S Sharma Sushma S Johansson Erik E Chabes Andrei A Xu Rui-Ming RM Zhang Zhiguo Z

Science (New York, N.Y.) 20180816 6409

How parental histone (H3-H4)<sub>2</sub> tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands of DNA replication forks for epigenetic inheritance remains elusive. Here we show that parental (H3-H4)<sub>2</sub> tetramers are assembled into nucleosomes onto both leading and lagging strands, with a slight preference for lagging strands. The lagging-strand preference increases markedly in budding yeast cells lacking Dpb3 and Dpb4, two subunits ...[more]

PMID: 30115745

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Asymmetric partition of parental histone (H3-H4)2 tetramers onto replicating DNA strands

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