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Bacterial Factors Required for Transmission of Streptococcus pneumoniae in Mammalian Hosts.


ABSTRACT: The capacity of Streptococcus pneumoniae to successfully transmit and colonize new human hosts is a critical aspect of pneumococcal population biology and a prerequisite for invasive disease. However, the bacterial mechanisms underlying this process remain largely unknown. To identify bacterial factors required for transmission, we conducted a high-throughput genetic screen with a transposon sequencing (Tn-seq) library of a pneumococcal strain in a ferret transmission model. Key players in both metabolism and transcriptional regulation were identified as required for efficient bacterial transmission. Targeted deletion of the putative C3-degrading protease CppA, iron transporter PiaA, or competence regulatory histidine kinase ComD significantly decreased transmissibility in a mouse model, further validating the screen. Maternal vaccination with recombinant surface-exposed PiaA and CppA alone or in combination blocked transmission in offspring and were more effective than capsule-based vaccines. These data underscore the possibility of targeting pneumococcal transmission as a means of eliminating invasive disease in the population.

SUBMITTER: Rowe HM 

PROVIDER: S-EPMC6598203 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Bacterial Factors Required for Transmission of Streptococcus pneumoniae in Mammalian Hosts.

Rowe Hannah M HM   Karlsson Erik E   Echlin Haley H   Chang Ti-Cheng TC   Wang Lei L   van Opijnen Tim T   Pounds Stanley B SB   Schultz-Cherry Stacey S   Rosch Jason W JW  

Cell host & microbe 20190521 6


The capacity of Streptococcus pneumoniae to successfully transmit and colonize new human hosts is a critical aspect of pneumococcal population biology and a prerequisite for invasive disease. However, the bacterial mechanisms underlying this process remain largely unknown. To identify bacterial factors required for transmission, we conducted a high-throughput genetic screen with a transposon sequencing (Tn-seq) library of a pneumococcal strain in a ferret transmission model. Key players in both  ...[more]

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