Ontology highlight
ABSTRACT:
SUBMITTER: Wu Y
PROVIDER: S-EPMC6599060 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Wu Yi Y Zhang Jing J Peng Boya B Tian Dan D Zhang Dong D Li Yang Y Feng Xiaoyu X Liu Jinghao J Li Jun J Zhang Teng T Liu Xiaoyong X Lu Jing J Chen Baian B Wang Songlin S
Nature communications 20190628 1
A substantial number of mouse genes, about 25%, are embryonically lethal when knocked out. Using current genetic tools, such as the CRISPR-Cas9 system, it is difficult-or even impossible-to produce viable mice with heritable embryonically lethal mutations. Here, we establish a one-step method for microinjection of CRISPR reagents into one blastomere of two-cell embryos to generate viable chimeric founder mice with a heritable embryonically lethal mutation, of either Virma or Dpm1. By examining f ...[more]