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Heritable genome editing in C. elegans via a CRISPR-Cas9 system.

ABSTRACT: We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

SUBMITTER: Friedland AE

PROVIDER: S-EPMC3822328 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Heritable genome editing in C. elegans via a CRISPR-Cas9 system.

Friedland Ari E AE Tzur Yonatan B YB Esvelt Kevin M KM Colaiácovo Monica P MP Church George M GM Calarco John A JA

Nature methods 20130630 8

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants. ...[more]

PMID: 23817069

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