Ontology highlight
ABSTRACT:
SUBMITTER: Friedland AE
PROVIDER: S-EPMC3822328 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Friedland Ari E AE Tzur Yonatan B YB Esvelt Kevin M KM Colaiácovo Monica P MP Church George M GM Calarco John A JA
Nature methods 20130630 8
We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants. ...[more]