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Intrinsic role of coagulase negative staphylococci norA-like efflux system in fluoroquinolones resistance.


ABSTRACT: NorA is a Staphylococcus aureus multidrug transporter that exports structurally distinct compounds including fluoroquinolones. In this study norA-like genes of Staphylococcus epidermidis (norA SEP) and Staphylococcus haemolyticus (norA SHAE) were identified and sequenced. The nucleotide identity of norA SEP and norA SHAE with norA was 75.3 and 74.1%, respectively, and the amino acid identity 87.7 and 86%, respectively. Inactivation of norA SEP increased the ciprofloxacin susceptibility of E. coli DH5? carrying the pB SK 198 norA SEP EZ cat norA SEP plasmid.

SUBMITTER: Marco L 

PROVIDER: S-EPMC6604952 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Intrinsic role of coagulase negative staphylococci <i>norA</i>-like efflux system in fluoroquinolones resistance.

Marco Ligozzi L   Liliana Galia G   Anna Bertoncelli B   Annarita Mazzariol M  

AIMS microbiology 20171114 4


NorA is a <i>Staphylococcus aureus</i> multidrug transporter that exports structurally distinct compounds including fluoroquinolones. In this study <i>norA-</i>like genes of <i>Staphylococcus</i> <i>epidermidis</i> (<i>norA</i> <sub>SEP</sub>) and <i>Staphylococcus haemolyticus</i> (<i>norA</i> <sub>SHAE</sub>) were identified and sequenced. The nucleotide identity of <i>norA</i> <sub>SEP</sub> and <i>norA</i> <sub>SHAE</sub> with <i>norA</i> was 75.3 and 74.1%, respectively, and the amino acid  ...[more]

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