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Genomic analysis of serologically untypable human enteroviruses in Taiwan.

ABSTRACT: BACKGROUND:Human enteroviruses contain over 100 serotypes. We have routinely conducted enterovirus surveillance in northern Taiwan; but about 10% of isolates could not be serotyped using traditional assays. Next-generation sequencing (NGS) is a powerful tool for genome sequencing. METHODS:In this study, we established an NGS platform to conduct genome sequencing for the serologically untypable enterovirus isolates. RESULTS:Among 130 serologically untypable isolates, 121 (93%) of them were classified into 29 serotypes using CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer)-based RT-PCR to amplify VP1 genes (VP1-CODEHOP). We further selected 52 samples for NGS and identified 59 genome sequences from 51 samples, including 8 samples containing two virus genomes. We also detected 23 genome variants (nucleotide identity

SUBMITTER: Chien YS

PROVIDER: S-EPMC6607526 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Genomic analysis of serologically untypable human enteroviruses in Taiwan.

Chien Yeh-Sheng YS Luo Shu-Ting ST Tsao Kuo-Chien KC Huang Yhu-Chering YC Chung Wan-Yu WY Liao Yu-Chieh YC Tan Yi Y Das Suman R SR Lee Min-Shi MS

Journal of biomedical science 20190703 1

<h4>Background</h4>Human enteroviruses contain over 100 serotypes. We have routinely conducted enterovirus surveillance in northern Taiwan; but about 10% of isolates could not be serotyped using traditional assays. Next-generation sequencing (NGS) is a powerful tool for genome sequencing.<h4>Methods</h4>In this study, we established an NGS platform to conduct genome sequencing for the serologically untypable enterovirus isolates.<h4>Results</h4>Among 130 serologically untypable isolates, 121 (93 ...[more]

PMID: 31266491

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