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NK1.1+ cells promote sustained tissue injury and inflammation after trauma with hemorrhagic shock.


ABSTRACT: Various cell populations expressing NK1.1 contribute to innate host defense and systemic inflammatory responses, but their role in hemorrhagic shock and trauma remains uncertain. NK1.1+ cells were depleted by i.p. administration of anti-NK1.1 (or isotype control) on two consecutive days, followed by hemorrhagic shock with resuscitation and peripheral tissue trauma (HS/T). The plasma levels of IL-6, MCP-1, alanine transaminase (ALT), and aspartate aminotransferase (AST) were measured at 6 and 24 h. Histology in liver and gut were examined at 6 and 24 h. The number of NK cells, NKT cells, neutrophils, and macrophages in liver, as well as intracellular staining for TNF-?, IFN-?, and MCP-1 in liver cell populations were determined by flow cytometry. Control mice subjected to HS/T exhibited end organ damage manifested by marked increases in circulating ALT, AST, and MCP-1 levels, as well as histologic evidence of hepatic necrosis and gut injury. Although NK1.1+ cell-depleted mice exhibited a similar degree of organ damage as nondepleted animals at 6 h, NK1.1+ cell depletion resulted in marked suppression of both liver and gut injury by 24 h after HS/T. These findings indicate that NK1.1+ cells contribute to the persistence of inflammation leading to end organ damage in the liver and gut.

SUBMITTER: Chen S 

PROVIDER: S-EPMC6608053 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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NK1.1<sup>+</sup> cells promote sustained tissue injury and inflammation after trauma with hemorrhagic shock.

Chen Shuhua S   Hoffman Rosemary A RA   Scott Melanie M   Manson Joanna J   Loughran Patricia P   Ramadan Mostafa M   Demetris Anthony J AJ   Billiar Timothy R TR  

Journal of leukocyte biology 20170517 1


Various cell populations expressing NK1.1 contribute to innate host defense and systemic inflammatory responses, but their role in hemorrhagic shock and trauma remains uncertain. NK1.1<sup>+</sup> cells were depleted by i.p. administration of anti-NK1.1 (or isotype control) on two consecutive days, followed by hemorrhagic shock with resuscitation and peripheral tissue trauma (HS/T). The plasma levels of IL-6, MCP-1, alanine transaminase (ALT), and aspartate aminotransferase (AST) were measured a  ...[more]

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