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Kidney and organoid single-cell transcriptomics: the end of the beginning.

ABSTRACT: Single-cell RNA sequencing (scRNA-seq) technologies are increasingly being applied to reveal cellular heterogeneity in kidney development and disease. In just the last year, multiple scRNA-seq datasets have been generated from kidney organoids, developing mouse and human kidney, adult kidney, and kidney cancer. The data generated enables a much deeper understanding of biological processes within and between cells. It has also elucidated unforeseen cell lineage relationships, defined the presence of off-target cell types in kidney organoids, and revealed a diverse inflammatory response in a human kidney allograft undergoing rejection. This review summarizes the recent rapid progress in scRNA-seq of the kidney and outlines future directions for single-cell technologies as applied to the kidney.

SUBMITTER: Wilson PC

PROVIDER: S-EPMC6609508 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Kidney and organoid single-cell transcriptomics: the end of the beginning.

Wilson Parker C PC Humphreys Benjamin D BD

Pediatric nephrology (Berlin, Germany) 20190104 2

Single-cell RNA sequencing (scRNA-seq) technologies are increasingly being applied to reveal cellular heterogeneity in kidney development and disease. In just the last year, multiple scRNA-seq datasets have been generated from kidney organoids, developing mouse and human kidney, adult kidney, and kidney cancer. The data generated enables a much deeper understanding of biological processes within and between cells. It has also elucidated unforeseen cell lineage relationships, defined the presence ...[more]

PMID: 30607565

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