?2-Chimaerin is essential for neural stem cell homeostasis in mouse adult neurogenesis.

ABSTRACT: Adult hippocampal neurogenesis involves the lifelong generation of neurons. The process depends on the homeostasis of the production of neurons and maintenance of the adult neural stem cell (NSC) pool. Here, we report that ?2-chimaerin, a Rho GTPase-activating protein, is essential for NSC homeostasis in adult hippocampal neurogenesis. Conditional deletion of ?2-chimaerin in adult NSCs resulted in the premature differentiation of NSCs into intermediate progenitor cells (IPCs), which ultimately depleted the NSC pool and impaired neuron generation. Single-cell RNA sequencing and pseudotime analyses revealed that ?2-chimaerin-conditional knockout (?2-CKO) mice lacked a unique NSC subpopulation, termed Klotho-expressing NSCs, during the transition of NSCs to IPCs. Furthermore, ?2-CKO led to defects in hippocampal synaptic plasticity and anxiety/depression-like behaviors in mice. Our findings collectively demonstrate that ?2-chimaerin plays an essential role in adult hippocampal NSC homeostasis to maintain proper brain function.

SUBMITTER: Su YT 

PROVIDER: S-EPMC6613132 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature