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Cytotoxicity and proinflammatory effects of titanium and zirconia particles.

ABSTRACT: BACKGROUND:To assess the effects of differently sized titanium (Ti) and zirconia (Zr) particles on (1) the metabolic activity of osteosarcoma-derived osteoblasts (SaOs-2) and human gingival fibroblasts (HGF) and (2) the cytokine expression of monocytes (THP-1) METHODS: Ti (60-80?nm and 100?nm) and Zr (2??m and 75??m) particles were incubated with SaOs-2, HGF, and THP-1 cells. At days 0, 2, 4, and 7 and 0, 1, 2, and 4 (THP-1), the mitochondrial activity was assessed and enzyme-linked immunosorbent assays were used to determine interleukin (IL)-1 beta and IL-6 concentrations of stimulated THP-1 at day 1. RESULTS:Ti60-80, Ti100, Zr2, and Zr75 particles were associated with gradual and significant within-group decreases in the viability of SaOs-2 and HGF cells. These effects were less pronounced in the Zr group. Similar to control cells, THP-1 did not reveal any significant increases in IL-1 beta and IL-6 concentrations. Viability of THP-1 was merely impaired in the presence of Ti100. CONCLUSIONS:Ti and Zr particles had a detrimental effect on the viability of SaOs-2 and HGF, but no proinflammatory effect on THP-1.

SUBMITTER: Schwarz F

PROVIDER: S-EPMC6614223 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Cytotoxicity and proinflammatory effects of titanium and zirconia particles.

Schwarz Frank F Langer Maike M Hagena Tina T Hartig Brigitte B Sader Robert R Becker Jürgen J

International journal of implant dentistry 20190709 1

<h4>Background</h4>To assess the effects of differently sized titanium (Ti) and zirconia (Zr) particles on (1) the metabolic activity of osteosarcoma-derived osteoblasts (SaOs-2) and human gingival fibroblasts (HGF) and (2) the cytokine expression of monocytes (THP-1) METHODS: Ti (60-80 nm and 100 nm) and Zr (2 μm and 75 μm) particles were incubated with SaOs-2, HGF, and THP-1 cells. At days 0, 2, 4, and 7 and 0, 1, 2, and 4 (THP-1), the mitochondrial activity was assessed and enzyme-linked immu ...[more]

PMID: 31286286

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Project description:Statement of problemTo separate the crown from the titanium base abutment, by using heat, caused oxidization of the titanium base abutment. The effect of this procedure on the retention of a crown is unclear.PurposeTo compare the resin bond strength and failure type between zirconia crowns and titanium base abutments utilizing four different surface treatments. Surface roughness and morphology of each surface treatment were also investigated.Material and methodsForty titanium base abutments (Variobase®) were divided into four groups, 1. Control, 2. Air abraded, 3. Oxidized, and 4. Oxidized-air abraded. Oxidized and oxidized-air abraded groups were debonded from zirconia crowns using constant dry heat at 500 ˚C. For air abraded and oxidized-air abraded groups (after oxidization), the titanium base abutments were air abraded with Al3O2. After cleaning, one specimen of each group was investigated under a non-contact profilometer (50x), then the same samples were investigated under SEM at 25,300,500,1000 magnification and EDS at 30kV of accelerated voltage. All specimens were then cemented (RelyX Ultimate). After aging, with thermocycling under 5C° to 55C°,120 seconds dwell time for 5,000 cycles, bond strength was tested and statistical differences were calculated with One-way ANOVA (p-value <0.05) follow by Tukey test. All separated crowns and titanium base abutments were investigated under a light microscope (20x), using fisher's exact test for correlation of the failure types.ResultsThere was a significant difference in the mean value of tensile bond strength among the control and test groups. Comparisons between control(237.6±46.3N) and oxidized(241.7±46.3N) showed statistically different values from air abraded(372.9±113.2N) when assembled using different surface treatments of the titanium-based abutments. (p-value<0.005) As for failure type, there were statistically significant differences between control versus air abraded, control versus oxidized-air abraded, oxidized versus air abraded, and oxidized versus oxidized-air abraded. (p-value<0.001) The titanium surface morphology shown from the profilometer and SEM was coordinated. Control (Ra 333.8nm) and oxidized (Ra 321.0nm) groups surfaces showed smooth, corrugated surfaces, meanwhile air abraded (Ra 476.0nm) and oxidized-air abraded (Ra 423.8nm) groups showed rough, rugged surfaces.ConclusionHeat oxidization of titanium-based abutments did not adversely affect tensile bond strength or the failure mode and surface roughness between titanium base abutments and zirconia crowns. However, air abrasion of the titanium surface increased surface roughness and retentive strength.Clinical implicationsThe titanium base abutments that were oxidized under heat treatment did not have an effect on crown retention. Thoroughly air abraded the titanium abutment prior to cementation can increase cement bond strength.

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Cytotoxicity and proinflammatory effects of titanium and zirconia particles.

Publications

Cytotoxicity and proinflammatory effects of titanium and zirconia particles.

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