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An in vitro chicken gut model for the assessment of phytase producing bacteria.


ABSTRACT: An in vitro simulated chicken gut model was proposed for studying the phytase activity of selected bacteria such as Streptococcus thermophilus, Sporosarcina pasteurii, Sporosarcina globispora, and Sporosarcina psychrophila using known probiotic bacterium, Lactobacillus helveticus as a control. The selected bacteria were viable in the intestinal lumen and produced extracellular phytase at optimal phytate concentration of 6.25 mM when compared to 3.125 mM and 12.5 mM. These bacteria demonstrated significantly higher (p??0.05) at the tested pH. Among the analyzed bacteria, the members of the genus Sporosarcina showed better phytate degradation when compared to S. thermophilus. The proposed model can be extended to analyze any extracellular enzymes produced by gut microbes.

SUBMITTER: Priyodip P 

PROVIDER: S-EPMC6614281 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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An in vitro chicken gut model for the assessment of phytase producing bacteria.

Priyodip Paul P   Balaji Seetharaman S  

3 Biotech 20190708 8


An in vitro simulated chicken gut model was proposed for studying the phytase activity of selected bacteria such as <i>Streptococcus thermophilus</i>, <i>Sporosarcina pasteurii</i>, <i>Sporosarcina globispora</i>, and <i>Sporosarcina psychrophila</i> using known probiotic bacterium, <i>Lactobacillus helveticus</i> as a control. The selected bacteria were viable in the intestinal lumen and produced extracellular phytase at optimal phytate concentration of 6.25 mM when compared to 3.125 mM and 12.  ...[more]

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