Ontology highlight
ABSTRACT:
SUBMITTER: Robb NC
PROVIDER: S-EPMC6614853 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Robb Nicole C NC Te Velthuis Aartjan J W AJW Fodor Ervin E Kapanidis Achillefs N AN
Nucleic acids research 20190701 12
The viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; further, little is known about cRNA promoter conformations. To probe the RNA conformations adopted during initial replication, we monitored single, surface-immobilized vRNA and cRNA ...[more]