Dataset Information

Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0.

ABSTRACT: The limited per-pixel bandwidth of most microscopy methods requires compromises between field of view, sampling density and imaging speed. This limitation constrains studies involving complex motion or fast cellular signaling, and presents a major bottleneck for high-throughput structural imaging. Here, we combine high-speed intensified camera technology with a versatile, reconfigurable and dramatically improved Swept, Confocally Aligned Planar Excitation (SCAPE) microscope design that can achieve high-resolution volumetric imaging at over 300 volumes per second and over 1.2?GHz pixel rates. We demonstrate near-isotropic sampling in freely moving Caenorhabditis elegans, and analyze real-time blood flow and calcium dynamics in the beating zebrafish heart. The same system also permits high-throughput structural imaging of mounted, intact, cleared and expanded samples. SCAPE 2.0's significantly lower photodamage compared to point-scanning techniques is also confirmed. Our results demonstrate that SCAPE 2.0 is a powerful, yet accessible imaging platform for myriad emerging high-speed dynamic and high-throughput volumetric microscopy applications.

SUBMITTER: Voleti V

PROVIDER: S-EPMC6885017 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0.

Voleti Venkatakaushik V Patel Kripa B KB Li Wenze W Perez Campos Citlali C Bharadwaj Srinidhi S Yu Hang H Ford Caitlin C Casper Malte J MJ Yan Richard Wenwei RW Liang Wenxuan W Wen Chentao C Kimura Koutarou D KD Targoff Kimara L KL Hillman Elizabeth M C EMC

Nature methods 20190927 10

The limited per-pixel bandwidth of most microscopy methods requires compromises between field of view, sampling density and imaging speed. This limitation constrains studies involving complex motion or fast cellular signaling, and presents a major bottleneck for high-throughput structural imaging. Here, we combine high-speed intensified camera technology with a versatile, reconfigurable and dramatically improved Swept, Confocally Aligned Planar Excitation (SCAPE) microscope design that can achie ...[more]

PMID: 31562489

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Dataset Information

Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0.

Publications

Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0.

Similar Datasets

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