Dataset Information

Inhibition of Bcl6b promotes gastric cancer by amplifying inflammation in mice.

ABSTRACT:

Background

Chronic gastritis has been demonstrated to be a key cause of gastric cancer (GC), and control of gastric inflammation is regarded as an effective treatment for the clinical prevention of gastric carcinogenesis. However, there remains an unmet need to identify the dominant regulators of gastric oncogenesis-associated inflammation in vivo.

Methods

The mouse model for the study of inflammation-associated GC was induced by Benzo[a]pyrene (BaP) intragastric administration in Bcl6b^-/- and wildtype mice on a C57BL/6 background. 5-Aza-2'-deoxycytidine (5-Aza), the demethylation drug, was intraperitoneally injected to restore Bcl6b expression. Human GC tissue array was used to analyse patient survival based on BCL6B and CD3 protein expression.

Results

Bcl6b was gradually downregulated by its own promoter hypermethylation in parallel to an increasing inflammatory response during the progression of BaP-induced gastric carcinogenesis in mice. Moreover, knockout of Bcl6b dramatically worsened the severity of gastric cancer and aggravated the inflammatory response in the BaP-induced mice GC model. Re-activation of Bcl6b by 5-Aza impeded inflammatory amplification and BaP-induced GC development, prolonging survival time in wildtype mice, whereas no notable curative effect occurred in Bcl6b^-/- mice with 5-Aza treatment. Finally, significant negative correlations were detected between the mRNA levels of BCL6B and inflammatory cytokines in human GC tissues; patients harbouring BCL6B-negetive and severe-inflammation GC tumours were found to exhibit the shortest survival time.

Conclusions

Epigenetic inactivation of Bcl6b promotes gastric cancer through amplification of the gastric inflammatory response in vivo and offers a new approach for GC treatment and regenerative medicine.

SUBMITTER: Cai WY

PROVIDER: S-EPMC6617686 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Publications

Inhibition of Bcl6b promotes gastric cancer by amplifying inflammation in mice.

Cai Wang-Yu WY Lin Ling-Yun LY Wang Lin L Yang Li L Ye Guo-Dong GD Zeng Qiang Q Cheng Jia J Xie Yuan-Yuan YY Chen Mao-Li ML Luo Qi-Cong QC

Cell communication and signaling : CCS 20190709 1

<h4>Background</h4>Chronic gastritis has been demonstrated to be a key cause of gastric cancer (GC), and control of gastric inflammation is regarded as an effective treatment for the clinical prevention of gastric carcinogenesis. However, there remains an unmet need to identify the dominant regulators of gastric oncogenesis-associated inflammation in vivo.<h4>Methods</h4>The mouse model for the study of inflammation-associated GC was induced by Benzo[a]pyrene (BaP) intragastric administration in ...[more]

PMID: 31288844

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Dataset Information

Inhibition of Bcl6b promotes gastric cancer by amplifying inflammation in mice.

Background

Methods

Results

Conclusions

Publications

Inhibition of Bcl6b promotes gastric cancer by amplifying inflammation in mice.

Similar Datasets

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