Unknown

Dataset Information

0

Spatial Positioning and Chemical Coupling in Coacervate-in-Proteinosome Protocells.


ABSTRACT: The integration of molecularly crowded microenvironments into membrane-enclosed protocell models represents a step towards more realistic representations of cellular structure and organization. Herein, the membrane diffusion-mediated nucleation of either negatively or positively charged coacervate microdroplets within the aqueous lumen of individual proteinosomes is used to prepare nested hybrid protocells with spatially organized and chemically coupled enzyme activities. The location and reconfiguration of the entrapped droplets are regulated by tuning the electrostatic interactions between the encapsulated coacervate and surrounding negatively charged proteinosome membrane. As a consequence, alternative modes of a cascade reaction involving membrane- and coacervate-segregated enzymes can be implemented within the coacervate-in-proteinosome protocells.

SUBMITTER: Booth R 

PROVIDER: S-EPMC6618027 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Spatial Positioning and Chemical Coupling in Coacervate-in-Proteinosome Protocells.

Booth Richard R   Qiao Yan Y   Li Mei M   Mann Stephen S  

Angewandte Chemie (International ed. in English) 20190522 27


The integration of molecularly crowded microenvironments into membrane-enclosed protocell models represents a step towards more realistic representations of cellular structure and organization. Herein, the membrane diffusion-mediated nucleation of either negatively or positively charged coacervate microdroplets within the aqueous lumen of individual proteinosomes is used to prepare nested hybrid protocells with spatially organized and chemically coupled enzyme activities. The location and reconf  ...[more]

Similar Datasets

| S-EPMC8163383 | biostudies-literature
| S-EPMC7722712 | biostudies-literature
| S-EPMC6851677 | biostudies-literature
| S-EPMC6128866 | biostudies-other
| S-EPMC6128941 | biostudies-literature
| S-EPMC6941959 | biostudies-literature
| S-EPMC6024302 | biostudies-literature
| S-EPMC8217494 | biostudies-literature
| S-EPMC7560875 | biostudies-literature
| S-EPMC4756681 | biostudies-literature