Dataset Information

Microbial spatial footprint as a driver of soil carbon stabilization.

ABSTRACT: Increasing the potential of soil to store carbon (C) is an acknowledged and emphasized strategy for capturing atmospheric CO₂. Well-recognized approaches for soil C accretion include reducing soil disturbance, increasing plant biomass inputs, and enhancing plant diversity. Yet experimental evidence often fails to support anticipated C gains, suggesting that our integrated understanding of soil C accretion remains insufficient. Here we use a unique combination of X-ray micro-tomography and micro-scale enzyme mapping to demonstrate for the first time that plant-stimulated soil pore formation appears to be a major, hitherto unrecognized, determinant of whether new C inputs are stored or lost to the atmosphere. Unlike monocultures, diverse plant communities favor the development of 30-150?µm pores. Such pores are the micro-environments associated with higher enzyme activities, and greater abundance of such pores translates into a greater spatial footprint that microorganisms make on the soil and consequently soil C storage capacity.

SUBMITTER: Kravchenko AN

PROVIDER: S-EPMC6635512 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Microbial spatial footprint as a driver of soil carbon stabilization.

Kravchenko A N AN Guber A K AK Razavi B S BS Koestel J J Quigley M Y MY Robertson G P GP Kuzyakov Y Y

Nature communications 20190716 1

Increasing the potential of soil to store carbon (C) is an acknowledged and emphasized strategy for capturing atmospheric CO<sub>2</sub>. Well-recognized approaches for soil C accretion include reducing soil disturbance, increasing plant biomass inputs, and enhancing plant diversity. Yet experimental evidence often fails to support anticipated C gains, suggesting that our integrated understanding of soil C accretion remains insufficient. Here we use a unique combination of X-ray micro-tomography ...[more]

PMID: 31311923

Similar Datasets

Project description:BackgroundTerrestrial ecosystems play a significant role in carbon (C) storage. Human activities, such as urbanization, infrastructure, and land use change, can reduce significantly the C stored in the soil. The aim of this research was to measure the spatial variability of soil organic C (SOC) in the national park La Malinche (NPLM) in the central highlands of Mexico as an example of highland ecosystems and to determine the impact of land use change on the SOC stocks through deterministic and geostatistical geographic information system (GIS) based methods.MethodsThe soil was collected from different landscapes, that is, pine, fir, oak and mixed forests, natural grassland, moor and arable land, and organic C content determined. Different GIS-based deterministic (inverse distance weighting, local polynomial interpolation and radial basis function) and geostatistical interpolation techniques (ordinary kriging, cokriging and empirical Bayes kriging) were used to map the SOC stocks and other environmental variables of the top soil layer.ResultsAll interpolation GIS-based methods described the spatial distribution of SOC of the NPLM satisfactorily. The total SOC stock of the NPLM was 2.45 Tg C with 85.3% in the forest (1.26 Tg C in the A horizon and 0.83 Tg C in the O horizon), 11.4% in the arable soil (0.23 Tg in the A horizon and only 0.05 Tg C in the O horizon) and 3.3% in the high moor (0.07 Tg C in the A horizon and <0.01 Tg C in the O horizon). The estimated total SOC stock in a preserved part of the forest in NPLM was 4.98 Tg C in 1938 and has nearly halved since then. Continuing this trend of converting all the remaining forest to arable land will decrease the total SOC stock to 0.52 Tg C.DiscussionDifferent factors explain the large variations in SOC stocks found in this study but the change in land use (conversion of forests into agricultural lands) was the major reason for the reduction of the SOC stocks in the high mountain ecosystem of the NPLM. Large amounts of C, however, could be stored potentially in this ecosystem if the area was used more sustainable. The information derived from this study could be used to recommend strategies to reverse the SOC loss in NPLM and other high-altitude temperate forests and sequester larger quantities of C. This research can serve as a reference for the analysis of SOC distribution in similar mountain ecosystems in central part of Mexico and in other parts of the world.

Project description:Soil microorganisms play fundamental roles in cycling of soil carbon, nitrogen, and other nutrients, yet we have a poor understanding of how soil microbiomes are shaped by their nutritional and physical environment. In this study, we investigated the successional dynamics of a soil microbiome during 21 weeks of enrichment on chitin and its monomer, N-acetylglucosamine. We examined succession of the soil communities in a physically heterogeneous soil matrix as well as a homogeneous liquid medium. The guiding hypothesis was that the initial species richness would influence the tendency for the selected consortia to stabilize and maintain a relatively constant community structure over time. We also hypothesized that long-term, substrate-driven growth would result in consortia with reduced species richness compared to the parent microbiome and that this process would be deterministic with relatively little variation between replicates. We found that the initial species richness does influence the long-term community stability in both liquid media and soil and that lower initial richness results in a more rapid convergence to stability. Despite use of the same soil inoculum and access to the same major substrate, the resulting community composition differed greatly in soil from that in liquid medium. Hence, distinct selective pressures in soils relative to homogenous liquid media exist and can control community succession dynamics. This difference is likely related to the fact that soil microbiomes are more likely to thrive, with fewer compositional changes, in a soil matrix than in liquid environments. IMPORTANCE The soil microbiome carries out important ecosystem functions, but interactions between soil microbial communities have been difficult to study due to the high microbial diversity and complexity of the soil habitat. In this study, we successfully obtained stable consortia with reduced complexity that contained species found in the original source soil. These consortia and the methods used to obtain them can be a valuable resource for exploration of specific mechanisms underlying soil microbial community ecology. The results of this study also provide new experimental context to better inform how soil microbial communities are shaped by new environments and how a combination of initial taxonomic structure and physical environment influences stability.

Project description:Returning crop residues is a possible practice for balancing soil carbon (C) loss. The turnover rate of organic C from crop residues to soil C is dependent on soil microbial community dynamics. However, the relationship between any temporal changes in the soil microbial community after crop straw inputs and the dynamics of straw-C distribution in the soil organic carbon (SOC) pool remains unclear. The present study investigated the allocation of straw-C into soil dissolved organic carbon (DOC), microbial biomass carbon (MBC), particulate organic carbon (POC) and mineral-associated organic carbon (MaOC) using stable isotope probing, as well as the temporal changes in the soil bacterial and fungal communities using high-throughput sequencing. After the first 180 days of straw decomposition, approximately 3.93% and 19.82% of straw-C was transformed into soil MaOC and POC, respectively, while 0.02% and 2.25% of straw-C was transformed into soil DOC and MBC, respectively. The temporal change of the soil microbial community was positively correlated with the dynamics of straw-C distribution to SOC (R > 0.5, P < 0.05). The copiotrophic bacteria (e.g., Streptomyces, Massilia and Sphingobacterium), cellulolytic bacteria and fungi (e.g., Dyella and Fusarium, Talaromyces), acidophilic bacteria (e.g., Edaphobacter and unclassified Acidobacteriaceae), denitrifying and N-fixing microbes (e.g., Burkholderia-Paraburkholderia, Paraphaeosphaeria and Bradyrhizobium), and fungi unclassified Sordariomycetes were significantly correlated with straw-C distribution to specific SOC fractions (P < 0.05), which explained more than 90% of the variation of straw-C allocation into soils. Copiotrophic, certain cellulolytic and denitrifying microbes had positively correlated with DOC- and MaOC-derived from straw, and other cellulolytic fungi (e.g., Talaromyces) and specific bacteria (e.g. Bradyrhizobium) were positively correlated with POC-derived from straw. Our results highlight that the temporal change of soil microbial community structure well reflects the conversion and distribution process of straw-C to SOC fractions.

Dataset Information

Microbial spatial footprint as a driver of soil carbon stabilization.

Publications

Microbial spatial footprint as a driver of soil carbon stabilization.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets