Ontology highlight
ABSTRACT:
SUBMITTER: Kappel K
PROVIDER: S-EPMC6636682 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Kappel Kalli K Liu Shiheng S Larsen Kevin P KP Skiniotis Georgios G Puglisi Elisabetta Viani EV Puglisi Joseph D JD Zhou Z Hong ZH Zhao Rui R Das Rhiju R
Nature methods 20181030 11
Increasingly, cryo-electron microscopy (cryo-EM) is used to determine the structures of RNA-protein assemblies, but nearly all maps determined with this method have biologically important regions where the local resolution does not permit RNA coordinate tracing. To address these omissions, we present de novo ribonucleoprotein modeling in real space through assembly of fragments together with experimental density in Rosetta (DRRAFTER). We show that DRRAFTER recovers near-native models for a diver ...[more]