Ontology highlight
ABSTRACT:
SUBMITTER: Martufi M
PROVIDER: S-EPMC6636881 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Martufi Matteo M Good Robert B RB Rapiteanu Radu R Schmidt Tobias T Patili Eleni E Tvermosegaard Ketil K New Maria M Nanthakumar Carmel B CB Betts Joanna J Blanchard Andy D AD Maratou Klio K
The CRISPR journal 20190201
Genome editing is a tool that has many applications, including the validation of potential drug targets. However, performing genome editing in low-passage primary human cells with the greatest physiological relevance is notoriously difficult. High editing efficiency is desired because it enables gene knockouts (KO) to be generated in bulk cellular populations and circumvents the problem of having to generate clonal cell isolates. Here, we describe a single-step workflow enabling >90% KO generati ...[more]