Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro.
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ABSTRACT:
SUBMITTER: Gonzalez S
PROVIDER: S-EPMC6637172 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
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Notch Inhibition Prevents Differentiation of Human Limbal Stem/Progenitor Cells in vitro.
González Sheyla S Uhm Heui H Deng Sophie X SX
Scientific reports 20190717 1
Notch signaling has been shown to regulate the homeostasis and wound healing of the corneal epithelium. We investigated the effect of Notch inhibition in the human limbal stem/progenitor cells (LSCs) in vitro by using small molecules. Treatment of the LSCs with DAPT and SAHM1 reduced the proliferation rate and maintained the undifferentiated state of the LSCs in a concentration dependent manner. Stratification and differentiation of the corneal epithelium were not reduced after Notch inhibition, ...[more]
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