Unknown

Dataset Information

0

The Tomato spotted wilt virus cell-to-cell movement protein (NSM ) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy.


ABSTRACT: Although the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum 'SR1' plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N.?benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM ) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N.?benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM ) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU). This is the first biological demonstration that Sw-5-mediated resistance is triggered by the TSWV NSM cell-to-cell movement protein.

SUBMITTER: Hallwass M 

PROVIDER: S-EPMC6638845 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

The Tomato spotted wilt virus cell-to-cell movement protein (NSM ) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy.

Hallwass Mariana M   de Oliveira Athos Silva AS   de Campos Dianese Erico E   Lohuis Dick D   Boiteux Leonardo Silva LS   Inoue-Nagata Alice Kazuko AK   Resende Renato O RO   Kormelink Richard R  

Molecular plant pathology 20140511 9


Although the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum 'SR1' plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N. benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive resp  ...[more]

Similar Datasets

| S-EPMC6638753 | biostudies-literature
| S-EPMC8402918 | biostudies-literature
| S-EPMC8828452 | biostudies-literature
2021-11-10 | PXD026246 | Pride
| S-EPMC7999277 | biostudies-literature
| S-EPMC4749231 | biostudies-literature
| S-EPMC6529424 | biostudies-literature
| S-EPMC7232525 | biostudies-literature
| S-EPMC6638320 | biostudies-literature
| S-EPMC8619209 | biostudies-literature