Dataset Information

Identification of a host protein interacting with Tomato spotted wilt virus ribonucleoproteins from Nicotiana benthamiana and yeast.

ABSTRACT: Viruses are obligate intracellular pathogens that depend on host factors to complete their infection cycle. Very little is known of which plant factors are required for successful Tomato spotted wilt orthotospovirus (TSWV) infection. The viral ribonucleoprotein (RNP) fraction from TSWV infected Nicotiana benthamiana plants was purified and its protein composition was analysed by proteomics by mass spectrometry to identify host proteins that co-purify with viral RNPs. Related, we expressed a TSWV replicon system in a non-host system, Bakers’ yeast (Saccharomyces cerevisiae), and purified as well the RNP fraction from yeast. Comparative proteomics was used to find common enriched proteins observed in both yeast and plant RNP fractions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Nicotiana Benthamiana Tomato Spotted Wilt Tospovirus Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD026246 | Pride | 2021-11-10

REPOSITORIES: Pride

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An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>.

Helderman Tieme A TA Deurhof Laurens L Bertran André A Boeren Sjef S Fokkens Like L Kormelink Richard R Joosten Matthieu H A J MHAJ Prins Marcel M van den Burg Harrold A HA

Viruses 20211030 11

The tripartite genome of the negative-stranded RNA virus <i>Tomato spotted wilt orthotospovirus</i> (TSWV) is assembled, together with two viral proteins, the nucleocapsid protein and the RNA-dependent RNA polymerase, into infectious ribonucleoprotein complexes (RNPs). These two viral proteins are, together, essential for viral replication and transcription, yet our knowledge on the host factors supporting these two processes remains limited. To fill this knowledge gap, the protein composition o ...[more]

PMID: 34834996

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			Action	DRS
	MQ_result_TH21-29.zip	Other
	MQ_result_TH31-36.zip	Other
	TH21_Yeast_RNP1.raw	Raw
	TH22_Yeast_RNP2.raw	Raw
	TH23_Yeast_RNP3.raw	Raw