Ontology highlight
ABSTRACT:
SUBMITTER: Boonekamp KE
PROVIDER: S-EPMC6642409 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Boonekamp Kim E KE Kretzschmar Kai K Wiener Dominique J DJ Asra Priyanca P Derakhshan Sepideh S Puschhof Jens J López-Iglesias Carmen C Peters Peter J PJ Basak Onur O Clevers Hans H
Proceedings of the National Academy of Sciences of the United States of America 20190628 29
Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an epidermal organoid culture system that allows long-term, genetically stable expansion of adult epidermal stem cells. Our epidermal expansion med ...[more]