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Forster Resonance Energy-Transfer-Based Ratiometric Fluorescent Indicator for Quantifying Fluoride Ion in Water and Toothpaste.

ABSTRACT: A Förster resonance energy-transfer (FRET)-based ratiometric fluorescent indicator Cou-FITC-Si toward fluoride ion has been designed and synthesized by combining coumarin unit and fluorescein derivative as energy donor and acceptor, respectively. The fluorescein unit is capped with tert-butyldiphenylchlorosilane. The indicator gives out emission responses based on switch-on of the FRET process that triggered by the desilylation mediated by the fluoride ion. The fluorescence emission spectrum of Cou-FITC-Si presents a significant bathochromic shift of 59 nm after the addition of fluoride ion with up to 180-fold increase of the fluorescence intensity ratio. The limit of detection of the Cou-FITC-Si indicator system toward fluoride ion was estimated to be 3.3 ppb. Furthermore, this indicator has been successfully applied for quantifying the fluoride ion of different concentrations from commercially available toothpaste.

SUBMITTER: Qiu B 

PROVIDER: S-EPMC6644089 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Förster Resonance Energy-Transfer-Based Ratiometric Fluorescent Indicator for Quantifying Fluoride Ion in Water and Toothpaste.

Qiu Bo B   Zeng Yi Y   Hu Rui R   Chen Leiyu L   Chen Jinping J   Yu Tianjun T   Yang Guoqiang G   Li Yi Y  

ACS omega 20181224 12

A Förster resonance energy-transfer (FRET)-based ratiometric fluorescent indicator <b>Cou-FITC-Si</b> toward fluoride ion has been designed and synthesized by combining coumarin unit and fluorescein derivative as energy donor and acceptor, respectively. The fluorescein unit is capped with <i>tert</i>-butyldiphenylchlorosilane. The indicator gives out emission responses based on switch-on of the FRET process that triggered by the desilylation mediated by the fluoride ion. The fluorescence emissio  ...[more]

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