Ontology highlight
ABSTRACT:
SUBMITTER: Nguyen TT
PROVIDER: S-EPMC7359203 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Small (Weinheim an der Bergstrasse, Germany) 20200406 17
A recent description of an antibody-free assay is significantly extended for small molecule analytes using allosteric transcription factors (aTFs) and Förster resonance energy transfer (FRET). The FRET signal indicates the differential binding of an aTF-DNA pair with a dose-dependent response to its effector molecule, i.e., the analyte. The new sensors described here, based on the well-characterized aTF TetR, demonstrate several new features of the assay approach: 1) the generalizability of the ...[more]