Dataset Information

High-Throughput Aqueous Two-Phase System Droplet Generation by Oil-Free Passive Microfluidics.

ABSTRACT: Aqueous two-phase system (ATPS) droplet generation has significant potential in biological and medical applications because of its excellent biocompatibility. However, the ultralow interfacial tension of ATPS makes droplet generation extremely challenging when compared with the conventional water-in-oil (W/O) system. In this paper, we passively produced ATPS droplets with a wide range of droplet size and high production rate without the involvement of an oil phase and external forces. For the first time, we reported important information of the flow rate and capillary (Ca) number for passive, oil-free ATPS droplet generation. It was found that the range of Ca numbers of the continuous phase under the jetting flow regime is 0.3-1.7, as compared to less than 0.1 in the W/O system, indicating the ultralow interfacial tension in ATPS. In addition, we successfully generated ATPS droplets with a radius as small as 7 ?m at the maximum frequency up to 300 Hz, which has not been achieved in previous studies. The size and generation frequency of ATPS droplets can be controlled independently by adjusting the inlet pressures and corresponding flow rates. We found that the droplet size is correlated with the pressure and flow rate ratios with the power-law exponents of 0.8 and 0.2, respectively.

SUBMITTER: Mastiani M

PROVIDER: S-EPMC6645416 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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High-Throughput Aqueous Two-Phase System Droplet Generation by Oil-Free Passive Microfluidics.

Mastiani Mohammad M Seo Seokju S Mosavati Babak B Kim Myeongsub M

ACS omega 20180816 8

Aqueous two-phase system (ATPS) droplet generation has significant potential in biological and medical applications because of its excellent biocompatibility. However, the ultralow interfacial tension of ATPS makes droplet generation extremely challenging when compared with the conventional water-in-oil (W/O) system. In this paper, we passively produced ATPS droplets with a wide range of droplet size and high production rate without the involvement of an oil phase and external forces. For the fi ...[more]

PMID: 31459062

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Project description:We have developed droplet microfluidic devices in thermoplastics and demonstrated the integration of key functional components that not only facilitate droplet generation, but also include electric field-assisted reagent injection, droplet splitting, and magnetic field-assisted bead extraction. We manufactured devices in poly(methyl methacrylate) and cyclic olefin polymer using a hot-embossing procedure employing silicon masters fabricated via photolithography and deep reactive ion etching techniques. Device characterization showed robust fabrication with uniform feature transfer and good embossing yield. Channel modification with heptadecafluoro-1,1,2,2-tetrahydrodecyltrichlorosilane increased device hydrophobicity, allowing stable generation of 330-pL aqueous droplets using T-junction configuration. Picoinjector and K-channel motifs were also both successfully integrated into the thermoplastic devices, allowing for robust control over electric field-assisted reagent injection, as well as droplet splitting with the K-channel. A magnetic field was also introduced to the K-channel geometry to allow for selective concentration of magnetic beads while decanting waste volume through droplet splitting. To show the ability to link multiple, modular features in a single thermoplastic device, we integrated droplet generation, reagent injection, and magnetic field-assisted droplet splitting on a single device, realizing a magnetic bead washing scheme to selectively exchange the fluid composition around the magnetic particles, analogous to the washing steps in many common biochemical assays. Finally, integrated devices were used to perform a proof-of-concept in-droplet β-galactosidase enzymatic assay combining enzyme-magnetic bead containing droplet generation, resorufin-β-D-galactopyranoside substrate injection, enzyme-substrate reaction, and enzyme-magnetic bead washing. By integrating multiple droplet operations and actuation forces we have demonstrated the potential of thermoplastic droplet microfluidic devices for complex (bio)chemical analysis, and we envision a path toward mass fabrication of droplet microfluidic devices for a range of (bio)chemical applications.

Dataset Information

High-Throughput Aqueous Two-Phase System Droplet Generation by Oil-Free Passive Microfluidics.

Publications

High-Throughput Aqueous Two-Phase System Droplet Generation by Oil-Free Passive Microfluidics.

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