Ontology highlight
ABSTRACT:
SUBMITTER: Gouridis G
PROVIDER: S-EPMC6656783 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Cell reports 20190701 3
The twin-ATPase ABCE1 has a vital function in mRNA translation by recycling terminated or stalled ribosomes. As for other functionally distinct ATP-binding cassette (ABC) proteins, the mechanochemical coupling of ATP hydrolysis to conformational changes remains elusive. Here, we use an integrated biophysical approach allowing direct observation of conformational dynamics and ribosome association of ABCE1 at the single-molecule level. Our results from FRET experiments show that the current static ...[more]