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Dok-3 deficient mice display different immune clustering and Tim-3 expression.

ABSTRACT:

Background

Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear.

Methods

We detected the expression of Tim-3 on spleen immune cells from Dok-3 deficient mice and control mice by flow cytometry.

Results

In this article, we found that Dok-3^-/- mice display almost entirely different immune clustering characteristics compared with wild type 129 mice. The CD4 T cells and CD8 T cells decreased and DC cells, macrophages, MDSCs increased when the Dok-3 gene knocked-out. The Tim-3 expression on CD4 T cells, CD8 T cells, NK cells, DC cells increased when the Dok-3 gene knocked-out. The macrophages and MDSCs just display the opposite results.

Conclusions

Although Dok-3^-/- mice display different immune clustering and Tim-3 expression, the mechanism still needs further study.

SUBMITTER: Yan W

PROVIDER: S-EPMC6660655 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Dok-3 deficient mice display different immune clustering and Tim-3 expression.

Yan Wenjiang W Tian Yijia Y Sun Peng P Yang Jingjing J Li Na N Sun Yuanyuan Y Wang Shuangxi S Zhang Cheng C

European journal of medical research 20190727 1

<h4>Background</h4>Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear.<h4>Methods</h4>We detected the expression of Tim-3 on spleen immune cells from Dok-3 deficient mice and control mice by flow cytometry.<h4>Results</h4>In this article, we found that Dok-3<sup>-/-</sup> mice display almost entirely different immune clustering characteristics c ...[more]

PMID: 31351483

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