Dataset Information

Biofilm Bridges Forming Structural Networks on Patterned Lubricant-Infused Surfaces.

ABSTRACT: Despite many decades of research, biofilm architecture and spreading mechanisms are still not clear because of the heterogenous 3D structure within biofilms. Here, patterned "slippery" lubricant-infused porous surfaces are utilized to study biofilm structure of Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Staphylococcus aureus. It is found that bacteria are able to spread over bacteria-repellent lubricant-infused regions by using a mechanism, termed "biofilm bridges". Here, it is demonstrated that bacteria use bridges to form interconnected networks between distant biofilm colonies. Detailed structure of bridges shows a spatial distribution of bacteria with an accumulation of respiratory active bacteria and biomass in the bridges. The core-shell structure of bridges formed by two-species mixed population is illustrated. It is demonstrated that eDNA and nutrients have a strong effect on biofilm bridges formation. Thus, it is believed that biofilm bridging is important to reveal the structure and communication within biofilms.

SUBMITTER: Lei W

PROVIDER: S-EPMC6662098 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Biofilm Bridges Forming Structural Networks on Patterned Lubricant-Infused Surfaces.

Lei Wenxi W Bruchmann Julia J Rüping Jan Lars JL Levkin Pavel A PA Schwartz Thomas T

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20190508 13

Despite many decades of research, biofilm architecture and spreading mechanisms are still not clear because of the heterogenous 3D structure within biofilms. Here, patterned "slippery" lubricant-infused porous surfaces are utilized to study biofilm structure of Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Staphylococcus aureus. It is found that bacteria are able to spread over bacteria-repellent lubricant-infused regions by using a mechanism, termed "biofilm bri ...[more]

PMID: 31380192

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Project description:Young's equation is fundamental to the concept of the wettability of a solid surface. It defines the contact angle for a droplet on a solid surface through a local equilibrium at the three-phase contact line. Recently, the concept of a liquid Young's law contact angle has been developed to describe the wettability of slippery liquid-infused porous surfaces (SLIPS) by droplets of an immiscible liquid. In this work, we present a new method to fabricate biphilic SLIP surfaces and show how the wettability of the composite SLIPS can be exploited with a macroscopic wedge-shaped pattern of two distinct lubricant liquids. In particular, we report the development of composite liquid surfaces on silicon substrates based on lithographically patterning a Teflon AF1600 coating and a superhydrophobic coating (Glaco Mirror Coat Zero), where the latter selectively dewets from the former. This creates a patterned base surface with preferential wetting to matched liquids: the fluoropolymer PTFE with a perfluorinated oil Krytox and the hydrophobic silica-based GLACO with olive oil (or other mineral oils or silicone oil). This allows us to successively imbibe our patterned solid substrates with two distinct oils and produce a composite liquid lubricant surface with the oils segregated as thin films into separate domains defined by the patterning. We illustrate that macroscopic wedge-shaped patterned SLIP surfaces enable low-friction droplet self-propulsion. Finally, we formulate an analytical model that captures the dependence of the droplet motion as a function of the wettability of the two liquid lubricant domains and the opening angle of the wedge. This allows us to derive scaling relationships between various physical and geometrical parameters. This work introduces a new approach to creating patterned liquid lubricant surfaces, demonstrates long-distance droplet self-propulsion on such surfaces, and sheds light on the interactions between liquid droplets and liquid surfaces.

Dataset Information

Biofilm Bridges Forming Structural Networks on Patterned Lubricant-Infused Surfaces.

Publications

Biofilm Bridges Forming Structural Networks on Patterned Lubricant-Infused Surfaces.

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