Ontology highlight
ABSTRACT:
SUBMITTER: Machado R
PROVIDER: S-EPMC6663088 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Machado Rosa R Bendesky Justin J Brown Madison M Spendier Kathrin K Hagen Guy M GM
Journal of imaging 20190704 7
Total internal reflection fluorescence microscopy with polarized excitation (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) primed with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE) coming into contact with a supported lipid bilayer containing mobile, monovalent DNP, modeling an immunological synapse. The spatial relationship of the IgE-bound high affinity IgE receptor (FcεRI) ...[more]