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Revisiting enteric methane emissions from domestic ruminants and their ?¹³C_CH4 source signature.

ABSTRACT: Accurate knowledge of ¹³C isotopic signature (?¹³C) of methane from each source is crucial for separating biogenic, fossil fuel and pyrogenic emissions in bottom-up and top-down methane budget. Livestock production is the largest anthropogenic source in the global methane budget, mostly from enteric fermentation of domestic ruminants. However, the global average, geographical distribution and temporal variations of the ?¹³C of enteric emissions are not well understood yet. Here, we provide a new estimation of C3-C4 diet composition of domestic ruminants (cattle, buffaloes, goats and sheep), a revised estimation of yearly enteric CH₄ emissions, and a new estimation for the evolution of its ?¹³C during the period 1961-2012. Compared to previous estimates, our results suggest a larger contribution of ruminants' enteric emissions to the increasing trend in global methane emissions between 2000 and 2012, and also a larger contribution to the observed decrease in the ?¹³C of atmospheric methane.

SUBMITTER: Chang J

PROVIDER: S-EPMC6668425 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Revisiting enteric methane emissions from domestic ruminants and their δ13CCH4 source signature.

Chang Jinfeng J Peng Shushi S Ciais Philippe P Saunois Marielle M Dangal Shree R S SRS Herrero Mario M Havlík Petr P Tian Hanqin H Bousquet Philippe P

Nature communications 20190731 1

Accurate knowledge of 13C isotopic signature (δ13C) of methane from each source is crucial for separating biogenic, fossil fuel and pyrogenic emissions in bottom-up and top-down methane budget. Livestock production is the largest anthropogenic source in the global methane budget, mostly from enteric fermentation of domestic ruminants. However, the global average, geographical distribution and temporal variations of the δ13C of enteric emissions are not well under ...[more]

PMID: 31366915

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Project description:Greenhouse gas (GHG) emissions from Arctic permafrost soils create a positive feedback loop of climate warming and further GHG emissions. Active methane uptake in these soils can reduce the impact of GHG on future Arctic warming potential. Aerobic methane oxidizers are thought to be responsible for this apparent methane sink, though Arctic representatives of these organisms have resisted culturing efforts. Here, we first used in situ gas flux measurements and qPCR to identify relative methane sink hotspots at a high Arctic cytosol site, we then labeled the active microbiome in situ using DNA Stable Isotope Probing (SIP) with heavy 13CH4 (at 100 ppm and 1000 ppm). This was followed by amplicon and metagenome sequencing to identify active organisms involved in CH4 metabolism in these high Arctic cryosols. Sequencing of 13C-labeled pmoA genes demonstrated that type II methanotrophs (Methylocapsa) were overall the dominant active methane oxidizers in these mineral cryosols, while type I methanotrophs (Methylomarinovum) were only detected in the 100 ppm SIP treatment. From the SIP-13C-labeled DNA, we retrieved nine high to intermediate quality metagenome-assembled genomes (MAGs) belonging to the Proteobacteria, Gemmatimonadetes, and Chloroflexi, with three of these MAGs containing genes associated with methanotrophy. A novel Chloroflexi MAG contained a mmoX gene along with other methane oxidation pathway genes, identifying it as a potential uncultured methane oxidizer. This MAG also contained genes for copper import, synthesis of biopolymers, mercury detoxification, and ammonia uptake, indicating that this bacterium is strongly adapted to conditions in active layer permafrost and providing new insights into methane biogeochemical cycling. In addition, Betaproteobacterial MAGs were also identified as potential cross-feeders with methanotrophs in these Arctic cryosols. Overall, in situ SIP labeling combined with metagenomics and genome binning demonstrated to be a useful tool for discovering and characterizing novel organisms related to specific microbial functions or biogeochemical cycles of interest. Our findings reveal a unique and active Arctic cryosol microbial community potentially involved in CH4 cycling.

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Revisiting enteric methane emissions from domestic ruminants and their ?¹³C_CH4 source signature.

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Revisiting enteric methane emissions from domestic ruminants and their δ<sup>13</sup>C<sub>CH4</sub> source signature.

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Dataset Information

Revisiting enteric methane emissions from domestic ruminants and their ?13CCH4 source signature.

Publications

Revisiting enteric methane emissions from domestic ruminants and their δ<sup>13</sup>C<sub>CH4</sub> source signature.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Revisiting enteric methane emissions from domestic ruminants and their ?¹³C_CH4 source signature.