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Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome.

ABSTRACT: Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament.

SUBMITTER: Song X

PROVIDER: S-EPMC6675542 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome.

Song Xiyong X Shan Hong H Zhu Yanping Y Hu Shunlin S Xue Ling L Chen Yong Y Ding Wei W Niu Tongxin T Gu Jian J Ouyang Songying S Shen Qing-Tao QT Liu Zhi-Jie ZJ

eLife 20190710

Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed ...[more]

PMID: 31290740

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