A genome-wide profiling of brain DNA hydroxymethylation in Alzheimer's disease.

ABSTRACT:

Introduction

DNA methylation is a key epigenetic mechanism in brain aging and Alzheimer's disease (AD). The newly discovered 5-hydroxymethylcytosine mediates DNA demethylation, is highly abundant in the brain, and is dynamically regulated by life experiences. However, little is known about its genome-wide patterns and potential role in AD.

Methods

Using a genome-wide capture followed by high-throughput sequencing, we studied the genome-wide distribution of 5-hydroxymethylcytosine at specific genomic loci in human AD brain and identified differentially hydroxymethylated regions (DhMRs) associated with AD pathology.

Results

We identified 517 DhMRs significantly associated with neuritic plaques and 60 DhMRs associated with neurofibrillary tangles. DNA hydroxymethylation

SUBMITTER: Zhao J 

PROVIDER: S-EPMC6675576 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature