Dataset Information

Oral administration of Urtica macrorrhiza Hand.-Mazz. polysaccharides to protect against cyclophosphamide-induced intestinal immunosuppression.

ABSTRACT: As a strategy to prevent the well-known immunosuppressant effects of cyclophosphamide (CY), the immunomodulatory activity of the polysaccharide isolated from Urtica macrorrhiza Hand.-Mazz. (UMHMPS) was investigated in the present study. The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated. The immunomodulatory activity of UMHMPS was evaluated using a CY-induced immunosuppression mouse model. The results revealed that UMHMPS, which is composed of rhamnose, gluconic acid, galactose acid, galactose and xylose, exhibited potent immunomodulatory activity and low toxicity in mice. It increased the secretions of secretory immunoglobulin A, interferon (IFN)-? and interleukin (IL)-4, and maintained the balance of the ratios of IFN-?/IL-4 and cluster of differentiation (CD)3+/CD19+ cells in Peyer's patches. Furthermore, it increased the expression of Toll-like receptor (TLR)-4, indicating that TLR4 may be one of the receptors of UMHMPS. Therefore, the present study provides evidence for the potential use of UMHMPS as an immune enhancement drug in chemotherapy.

SUBMITTER: Wang Z

PROVIDER: S-EPMC6676156 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Oral administration of <i>Urtica macrorrhiza</i> Hand.-Mazz. polysaccharides to protect against cyclophosphamide-induced intestinal immunosuppression.

Wang Zhongjuan Z Li Yanhua Y Wang Chongjing C Xia Hongying H Liang Yueqin Y Li Zhongkun Z

Experimental and therapeutic medicine 20190718 3

As a strategy to prevent the well-known immunosuppressant effects of cyclophosphamide (CY), the immunomodulatory activity of the polysaccharide isolated from <i>Urtica macrorrhiza</i> Hand.-Mazz. (UMHMPS) was investigated in the present study. The chemical properties of UMHMPS, including total carbohydrates, uronic acid, protein contents, monosaccharide compositions, molecular weight and structural confirmation, were investigated. The immunomodulatory activity of UMHMPS was evaluated using a CY- ...[more]

PMID: 31410170

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Project description:Lonicera macranthoides Hand.-Mazz (L. macranthoides) is a medicinal herb that is widely distributed in South China. The developmental stage and corolla dehiscence of the flower are the important factors affecting the quality of medicinal ingredients. However, neither the regulatory mechanism controlling chlorogenic acids biosynthesis in L. macranthoides nor the molecular basis of effect of corolla dehiscence on the quality of medicinal materials is fully understood. In this study, metabolomics and transcriptomics were used to analyze the metabolic and transcriptional differences of two different cultivars closed bud type (Bt), and flowering type (Ft), as well as the effect of jasmonic acid methyl ester (MeJA) on chlorogenic acids (CGAs) biosynthesis. In total, large number of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were filtered among three lines of samples. Gene metabolite correlation analyses revealed a 'core set' of 30 genes and 54 genes that were strongly correlated with CGAs biosynthesis and regulating the flowering, respectively. Quantitative real-time polymerase chain reaction results proved the alterations in the expression levels of genes encoding the pathways involved in CGAs biosynthesis. The ion abundances of CGAs were most significantly increased, while some of the CGAs derived and Caffeoyl-CoA-derived substances showed the most largely reduced abundances in the closed bud type (Bt) compared to the flowering type (Ft). MeJA may leads to the activation of downstream genes in CGAs biosynthesis pathway. Overall, there were significant differences in the transcriptional and metabolic levels of CGAs biosynthesis pathway in flower buds of different flowering cultivars. The redirection of metabolic flux may contribute to increased accumulation of CGAs. However, whether MeJA and flowering have direct effects on the accumulation of CGAs needs further studied. These researches effectively expanded the functional genomic library and provide new insights into CGAs biosynthesis in L. macranthoides.

Dataset Information

Oral administration of Urtica macrorrhiza Hand.-Mazz. polysaccharides to protect against cyclophosphamide-induced intestinal immunosuppression.

Publications

Oral administration of <i>Urtica macrorrhiza</i> Hand.-Mazz. polysaccharides to protect against cyclophosphamide-induced intestinal immunosuppression.

Similar Datasets

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