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Reliability of human cortical organoid generation.

ABSTRACT: The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.

SUBMITTER: Yoon SJ

PROVIDER: S-EPMC6677388 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Reliability of human cortical organoid generation.

Yoon Se-Jin SJ Elahi Lubayna S LS Pașca Anca M AM Marton Rebecca M RM Gordon Aaron A Revah Omer O Miura Yuki Y Walczak Elisabeth M EM Holdgate Gwendolyn M GM Fan H Christina HC Huguenard John R JR Geschwind Daniel H DH Pașca Sergiu P SP

Nature methods 20181220 1

The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for ...[more]

PMID: 30573846

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