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Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology.

ABSTRACT: Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein-protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.

SUBMITTER: Pedelacq JD

PROVIDER: S-EPMC6678664 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology.

Pedelacq Jean-Denis JD Cabantous Stéphanie S

International journal of molecular sciences 20190715 14

Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely ...[more]

PMID: 31311175

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