Ontology highlight
ABSTRACT:
SUBMITTER: Chen P
PROVIDER: S-EPMC6684407 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Chen Peng P Lam Kwok-Ho KH Liu Zheng Z Mindlin Frank A FA Chen Baohua B Gutierrez Craig B CB Huang Lan L Zhang Yongrong Y Hamza Therwa T Feng Hanping H Matsui Tsutomu T Bowen Mark E ME Perry Kay K Jin Rongsheng R
Nature structural & molecular biology 20190715 8
Clostridium difficile is an opportunistic pathogen that establishes in the colon when the gut microbiota are disrupted by antibiotics or disease. C. difficile infection (CDI) is largely caused by two virulence factors, TcdA and TcdB. Here, we report a 3.87-Å-resolution crystal structure of TcdB holotoxin that captures a unique conformation of TcdB at endosomal pH. Complementary biophysical studies suggest that the C-terminal combined repetitive oligopeptides (CROPs) domain of TcdB is dynamic and ...[more]