Ontology highlight
ABSTRACT:
SUBMITTER: Shellhammer JP
PROVIDER: S-EPMC6684483 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Shellhammer James P JP Pomeroy Amy E AE Li Yang Y Dujmusic Lorena L Elston Timothy C TC Hao Nan N Dohlman Henrik G HG
Yeast (Chichester, England) 20190627 8
The pheromone response pathway of the yeast Saccharomyces cerevisiae is a well-established model for the study of G proteins and mitogen-activated protein kinase (MAPK) cascades. Our longstanding ability to combine sophisticated genetic approaches with established functional assays has provided a thorough understanding of signalling mechanisms and regulation. In this report, we compare new and established methods used to quantify pheromone-dependent MAPK phosphorylation, transcriptional inductio ...[more]