Ontology highlight
ABSTRACT:
SUBMITTER: MacDougall MS
PROVIDER: S-EPMC6685429 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
MacDougall Matthew S MS Clarke Ryan R Merrill Bradley J BJ
Cell stem cell 20190516 2
Progression through states of pluripotency is required for cells in early mammalian embryos to transition away from heightened self-renewal and toward competency for lineage specification. Here, we use a CRISPR mutagenesis screen in mouse embryonic stem cells (ESCs) to identify unexpected roles for nuclear export and intracellular Ca<sup>2+</sup> homeostasis during the exit out of the naive state of pluripotency. Mutation of a plasma membrane Ca<sup>2+</sup> pump encoded by Atp2b1 increased intr ...[more]