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Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.

ABSTRACT: Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint. In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties. In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation. In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs). Results show high viability of MSCs within the IPN hydrogel, with improved mechanical properties compared to constructs comprised of individual components. These properties are further strengthened by integrating the hydrogel with a 3D woven structure. The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function. These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.

SUBMITTER: Moffat KL

PROVIDER: S-EPMC6687075 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.

Moffat Kristen L KL Goon Kelsey K Moutos Franklin T FT Estes Bradley T BT Oswald Sara J SJ Zhao Xuanhe X Guilak Farshid F

Macromolecular bioscience 20180724 10

Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint. In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties. In this regard, interpenetrating pol ...[more]

PMID: 30040175

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Project description:Composite industry has long been seeking practical solutions to boost laminate through-thickness strengths and interlaminar shear strengths (ILSS), so that composite primary structures, such as stiffeners, can bear higher complex loadings and be more delamination resistant. Three dimensional (3D) woven fabrics were normally employed to render higher transverse and shear strengths, but the difficulty and high expense in producing such fabrics make it a hard choice. Based on a novel idea that the warp yarns that interlock layers of the weft yarns might provide adequate fiber crimps that would allow the interlaminar shear or radial stresses to be transferred and borne by the fibers, rather than by the relatively weaker matrix resin, thus improving the transverse strengths, this work provided a two point five dimensional (2.5D) approach as a practical solution, and demonstrated the superior transverse performances of an economical 2.5D shallow-bend woven fabric (2.5DSBW) epoxy composites, over the conventional two dimensional (2D) laminates and the costly 3D counterpart composites. This approach also produced a potential candidate to fabricate high performance stiffeners, as shown by the test results of L-beams which are common structural components of any stiffeners. This study also discovered that an alternative structure, namely a 2.5D shallow-straight woven fabric (2.5DSSW), did not show any advantages over the two control structures, which were a 2D plain weave (2DPW) and a 3D orthogonal woven fabric (3DOW) made out of the same carbon fibers. Composites of these structures in this study were conveniently fabricated using a vacuum-assisted resin infusion process (VARI). The L-beams were tested using a custom-made test fixture. The strain distribution and failure mode analysis of these beams were conducted using Digital Image Correlation (DIC) and X-ray Computed Tomography Scanning (CT). The results demonstrated that the structures containing Z-yarns or having high yarn crimps or waviness, such as in cases of 3DOW and 2.5DSBW, respectively, were shown to withstand high loadings and to resist delamination, favorable for the applications of high-performance structural composites.

Dataset Information

Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.

Publications

Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.

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